Part:BBa_K3585003:Design

J23100-butyrate

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1240
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 6272
  Illegal BglII site found at 7408
  Illegal BglII site found at 7549
  Illegal XhoI site found at 1245
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In order to meet the typeIIS standard, we made a synonymous mutation of Asp50Asp and Leu137Leu to remove BsaI restriction site.

Source

The promoter sequence comes from Part:BBa_J23100. The biosynthetic gene cluster comes from Part:BBa_K3585001.